RESUMO
OBJECTIVE: To investigate the effects of acupuncture on JNK pathway and autophagy level in rats with intracerebral hemorrhage ï¼ICHï¼ and explore the partial mechanism of acupuncture against ICH. METHODS: SD rats were randomly divided into blank group, model group and acupuncture group. Each group was divided into Day 1, Day 3 and Day 7 subgroups respectively, with 5 rats in each group. The autologous blood injection was adopted to duplicate rat model of ICH. In the acupuncture group, the needle was inserted from "Baihui" ï¼GV20ï¼ towards "Qubin" ï¼GB7ï¼ on the affected side, stimulating for 30 min each time, once dailyï¼ the same acupuncture technique was opera-ted in each subgroup for 1, 3 and 7 days, separately. Using Bederson scale, the neurological deficit was evaluated in each group. Western blot was adopted to detect the protein expression levels of Beclin1, LC3â /â ¡, phosphorylated c-Jun amino-terminal kinase ï¼p-JNKï¼ and the phosphorylated ï¼pï¼-c-Jun around hematoma lesion of the brain tissue of rats in each group. RESULTS: After treatment, the neurological deficit score of rats in the model group was higher than that of the blank group at each time point ï¼P<0.05ï¼, and the score of the acupuncture group started declining since the 3rd day of treatment when compared with the model group ï¼P<0.05ï¼. At each time point, compared with the blank group, the protein expression levels of LC3â /â ¡, Beclin1, p-c-Jun and p-JNK was increased ï¼P<0.01ï¼. Compared with the model group, the protein expression level of LC3â /â ¡ was reduced ï¼P<0.05ï¼ï¼ the protein expression levels of Beclin1, p-c-Jun and p-JNK was increased ï¼P<0.05, P<0.01ï¼ on day 3 and 7 in the acupuncture group. CONCLUSION: Acupuncture can activate the JNK pathway in the brain tissue of rats with ICH and increase the level of autophagy, thereby improving the neurological function of the rats with ICH.
